Immunoaffinity isolation of Na,K-ATPase α3 isoform from pig kidney
نویسندگان
چکیده
منابع مشابه
Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3 Essential Role of Na,k-atpase Α3
1 A specific and essential role for Na,K-ATPase α3 in neurons co-expressing α1 and α3* Background: Neurons express two Na,K-ATPase isoforms, the ubiquitous α1 and neuron-specific α3. Result: α3 is important for control of membrane potential and is fully responsible for restoration of large [Na + ] i increases. Conclusion: α1 and α3 are required for basal neuronal function, but α3 controls resto...
متن کاملCognitive deficits caused by a disease-mutation in the α3 Na+/K+-ATPase isoform
The Na(+)/K(+)-ATPases maintain Na(+) and K(+) electrochemical gradients across the plasma membrane, a prerequisite for electrical excitability and secondary transport in neurons. Autosomal dominant mutations in the human ATP1A3 gene encoding the neuron-specific Na(+)/K(+)-ATPase α3 isoform cause different neurological diseases, including rapid-onset dystonia-parkinsonism (RDP) and alternating ...
متن کاملOuabain targets the Na+/K+-ATPase α3 isoform to inhibit cancer cell proliferation and induce apoptosis
Ouabain has been used for the treatment of heart failure and atrial fibrillation. Its potential anticancer effect has also attracted great interest. The aim of the present study was to evaluate the anticancer effect of ouabain and investigate its molecular target. The effects of ouabain on the viability of and induction of cellular death on OS-RC-2 renal cancer cells were examined using the MTT...
متن کاملRegulation of NaK-ATPase Activity by Neurotransmitters
The hitherto unknown NT-regulated mechanism of NaK-ATPase localized in the nerve ending membranes is found. The mechanism certainly has a functional significance and must be involved in the regulation – modulation of chemical synaptic transmission. On the other hand , the availability of the discovered specific protein, regulators (SFa and SFi) of synaptic origin, makes it possible to consider ...
متن کاملIsolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1989
ISSN: 0014-5793
DOI: 10.1016/0014-5793(89)81776-4